Article ID Journal Published Year Pages File Type
1172917 Analytical Biochemistry 2016 5 Pages PDF
Abstract

A simple, efficient, and reliable method is demonstrated for cloning long tandem arrays of the 601 nucleosomal positioning sequence. In addition, it is shown that such long arrays can be ligated together in vitro with high efficiency. By combining these two procedures it becomes straightforward to synthesize customized arrays that contain different (or variable) nucleosomal repeat lengths (NRLs) and monosome units bearing chemical modifications such as fluorophores, methyl groups, and reaction sites. This is, therefore, an enabling technology for the in vitro study of chromatin structure and function.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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