Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1173348 | Analytical Biochemistry | 2011 | 6 Pages |
Abstract
Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jong-Seo Kim, Mansup Shin, Jin-Su Song, Songhie An, Hie-Joon Kim,