Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1173379 | Analytical Biochemistry | 2015 | 7 Pages |
Abstract
Urinary levels of human serum albumin (hSA) fragment 408–423 have been proposed to represent an early marker for graft-versus-host disease (GvHD) and chronic kidney diseases. Here, we developed an enzyme-linked immunosorbent assay (ELISA) for the quantification of hSA(408–423). The sandwich ELISA has a detection limit of 0.5 ng/ml and is highly specific for hSA(408–423) because it does not cross-react with other albumin fragments or the full-length precursor. This ELISA allows rapid and convenient quantification of hSA(408–423) in bodily fluids, further clarifying the prognostic and diagnostic value of this peptide in GvHD, kidney disease, and other disorders.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Katharina B. Mohr, Onofrio Zirafi, Mark Hennies, Sebastian Wiese, Frank Kirchhoff, Jan Münch,