| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1173403 | Analytical Biochemistry | 2014 | 7 Pages |
Abstract
A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl-maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Marie Bøjstrup, Caspar Elo Christensen, Michael Skovbo Windahl, Anette Henriksen, Ole Hindsgaul,