| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1173464 | Analytical Biochemistry | 2014 | 5 Pages |
Abstract
We have prepared 4-nitrophenyl caffeate by a combination of standard procedures of organic synthesis and enzymatic deacetylation. Based on hydrolysis of 4-nitrophenyl caffeate, a convenient spectrophotometric assay was developed for specific monitoring of caffeoyl esterase. The method is fast and easy to perform, and it requires no expensive equipment. Its reliability was tested on eight enzyme preparations comprising various combinations of caffeoyl, feruloyl, and acetyl esterase as well as protease activities.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Andrej Chyba, Vladimír Mastihuba, Mária Mastihubová,