| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1174340 | Analytical Biochemistry | 2010 | 4 Pages |
Abstract
Arachidonic acid (AA) release is a central message in cell signaling. Fatty acid release is generally assessed by manual sampling of radioactivity release from cells prelabeled with a radiolabeled fatty acid. The assay is laborious, time-consuming, and susceptible to high noise. Here we present a fast and reproducible method for 96-well filter plates and cells in suspension, a method that is best suited for agonist concentration–response studies and, thus, for ligand screening. The method offers tremendous time and effort savings and enables execution of large experimental series previously unattainable for AA release studies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Pauli M. Turunen, Jaana Putula, Jyrki P. Kukkonen,