| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1174408 | Analytical Biochemistry | 2012 | 5 Pages |
Abstract
Because superoxide is involved in various physiological processes, many efforts have been made to improve its accurate quantification. We optimized and validated a superoxide-specific and -sensitive detection method. The protocol is based on fluorescence detection of the superoxide-specific hydroethidine (HE) oxidation product, 2-hydroxyethidium. We established a method for the quantification of superoxide production in isolated mitochondria without the need for acetone extraction and purification chromatography as described in previous studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Patricia Back, Filip Matthijssens, Jacques R. Vanfleteren, Bart P. Braeckman,