Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1174459 | Analytical Biochemistry | 2010 | 8 Pages |
Abstract
We report a new dendritic cell adhesion assay, using either immature or mature dendritic cells, for identifying functional dendritic cell-specific ICAM-3-grabbing nonintegrin (DC–SIGN) inhibitors. Because immature dendritic cells are responsible for pathogen binding and invasion, this in vitro assay provides an important link between in vitro results and pathogen-based in vivo assays. Furthermore, this assay does not require laborious expression, refolding, and purification of DC–SIGN carbohydrate recognition domain or extracellular domain as receptor-based assays. The assay power evaluated with Z and Z′ parameters enables screening of compound libraries and determination of IC50 values in the first stage of DC–SIGN inhibitor development.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nataša Obermajer, Urban Švajger, Matjaž Jeras, Sara Sattin, Anna Bernardi, Marko Anderluh,