Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1174851 | Analytical Biochemistry | 2010 | 5 Pages |
Abstract
We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1 M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato,