Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1175212 | Analytical Biochemistry | 2010 | 5 Pages |
Abstract
The ubiquitin–proteasome system (UPS) is a major degradation system for regulatory and misfolded proteins. UPS function has been implicated to exert a central role in the pathogenesis of various human diseases. Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle. We demonstrate that the specific proteasomal activity differs in individual muscle groups and decreases with aging. These findings warrant the use of appropriate controls and a careful interpretation of results in mammalian skeletal muscle pathologies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Karl-Heinz Strucksberg, Karthikeyan Tangavelou, Rolf Schröder, Christoph S. Clemen,