| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1175216 | Analytical Biochemistry | 2010 | 6 Pages |
Abstract
DNA with all cytosines, thymines, or all pyrimidines of one strand substituted by fluorescently labeled analogs shows diminished solubility in aqueous media and a strong tendency to aggregation that hampers enzymatic downstream processing. In this study, immobilization of fully fluorescently labeled DNA on microarrays was shown to resolve the named problems and to enable successive DNA degradation by exonuclease III. Fluorescence correlation spectroscopy and single-molecule counting for monitoring the course of DNA hydrolysis in real time revealed the virtually processive degradation of labeled DNA that occurred at an average rate of approximately 4 nt/s.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nicky Ehrlich, Katrin Anhalt, Christian Hübner, Susanne Brakmann,