| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1175477 | Analytical Biochemistry | 2008 | 6 Pages |
Abstract
A membrane-based high-throughput screening (HTS) assay for active d-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample. The method proposed can be used for quantitative DAAO determination in the range of 0.13 to 3.60 ng enzyme per probe. The protocol was successfully tested to screen a library of E. coli clones containing mutant DAAOs active toward target substrates.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Svetlana V. Khoronenkova, Vladimir I. Tishkov,