Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1175726 | Analytical Biochemistry | 2007 | 5 Pages |
Abstract
This article describes a new assay for isothermal enhancement of fluorescence intensity. The assay is based on the cleavage of duplexes formed by the chimeric DNA–rN1–DNA molecular beacon (cMB) and target DNA with Chlamydia pneumoniae RNase HII (CpRNase HII). The loop sequence of the cMB, which was designed according to the target sequence, contains a single ribonucleotide. The combination of CpRNase HII cleavage and cMB (RHMB) permitted a 90-fold increase in fluorescence intensity change compared with the hybridization reaction in the presence of the same amount of target DNA. These results indicate that the RHMB assay can enhance the fluorescence signal in real-time monitoring of the target DNA.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jingli Hou, Xipeng Liu, Jian Wang, Jianhua Liu, Tao Duan,