Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1175758 | Analytical Biochemistry | 2008 | 6 Pages |
Abstract
Capillary electrophoresis with contactless conductivity detection was used to directly quantify the ammonium produced in the enzymatic conversion of urea with urease. This allowed the characterization of the reaction without having to use more elaborate indirect optical methods for quantification. The maximum rate of reaction, Vmax, was determined as 5.1 mmol·mL−1·min−1, and the Michaelis–Menten constant, Km, was determined as 16 mM. Furthermore, the method was successfully applied to the determination of urea in clinical samples of human blood by using a conventional capillary and a microchip device.
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Authors
Aiping Schuchert-Shi, Peter C. Hauser,