Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1175970 | Analytical Biochemistry | 2007 | 5 Pages |
Abstract
A new analytical method based on capillary electrophoresis (CE) separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed for the determination of octopamine. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of octopamine. The separation and determination of the derivative was performed using a laboratory-built CE system with an optical fiber LED-induced fluorescence detector. Optimal separation was obtained at 20 kV using a background electrolyte solution consisting of 25 mM sodium borate (pH 9.2). High sensitivity detection was achieved by the optical fiber LED-induced fluorescence detection using a purple LED as the excitation source. The limit of detection (signal/noise = 3) for octopamine was 5.0 Ã 10â9 M. A calibration curve ranging from 1.0 Ã 10â8 to 5.0 Ã 10â7 M was shown to be linear. Using this method, the levels of octopamine in human plasma from healthy donors were determined.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qinglei Yu, Shulin Zhao, Fanggui Ye, Shuting Li,