Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1176134 | Analytical Biochemistry | 2006 | 6 Pages |
Abstract
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to develop a rapid and sensitive method for detection and quantitation of proteins in gels and on blots. The fluorescence response is quantitative for protein content between 10 ng and 20 μg per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shen Luo, Nancy B. Wehr, Rodney L. Levine,