Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1176384 | Analytical Biochemistry | 2008 | 5 Pages |
Abstract
In this report, we describe the construction and analysis of a cell-free protein synthesis system immobilized in calcium alginate microbeads. When incubated in a feeding solution that contained amino acids and other low-molecular-weight substrates, the microbeads transcribed and translated coimmobilized DNA into functional proteins. Protein synthesis continued for more than 15Â h with the diffusional supply of substrates and removal of by-products. In addition, functional proteins were generated from PCR-amplified genes as efficiently as from plasmid, suggesting that these cell-like microbeads could be used for functional screening of genomic libraries.
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Authors
Yong-Chan Kwon, Geun-Hee Hahn, Kang Moo Huh, Dong-Myung Kim,