Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1176483 | Analytical Biochemistry | 2007 | 6 Pages |
Abstract
A selective and sensitive gold nanoparticle-based electrochemical method for detection of hepatitis B virus DNA sequences was used. This method relies on the hybridization of amplified hepatitis B virus DNA strands with probes that are extended on paramagnetic beads. After separation of noncomplementary sequences, hybridized magnetic beads were treated with streptavidin-modified gold followed by silver enhancement. High selectivity and high sensitivity were obtained using electrochemical stripping detection of silver ions that were deposited on gold nanoparticles. With a signal/noise ratio of approximately 4.6, the detection limit was estimated to be 0.7 ng/ml.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
H. Hanaee, H. Ghourchian, A-A. Ziaee,