Article ID Journal Published Year Pages File Type
1176533 Analytical Biochemistry 2006 10 Pages PDF
Abstract

With the increasing use of capillary electrophoresis (CE) in the biotechnology industry, there is a demand for analytical tools and methodology that can be used to characterize CE profiles. This article describes the implementation and optimization of a robust online CE–mass spectrometry (CE–MS) system used for the characterization of several CE assays developed at Genentech Inc. These assays include CE as a complement to reverse-phase peptide mapping for the identification of small peptides eluting in the void volume, profiling N-linked glycopeptide heterogeneity, and determining O-linked site occupancy. In addition, CE–MS was used to confirm major 8-aminopyrene-1,3,6-trisulfonate (APTS)-labeled glycans released from recombinant antibodies that are routinely profiled by CE–laser-induced fluorescence (CE–LIF). For each study, CE–MS was able to successfully identify components seen in UV or LIF electropherograms, thereby expanding the capability of CE and CE–MS for profiling biomolecules.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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