| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1176646 | Analytical Biochemistry | 2006 | 6 Pages |
Abstract
An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shouzou Wei, James J. Brittin, John R. Falck, Siddam Anjaiah, Kasem Nithipatikom, Lijie Cui, William B. Campbell, Jorge H. Capdevila,