| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1176786 | Analytical Biochemistry | 2006 | 4 Pages |
Abstract
We have developed a simple isothermal (55 °C) reaction that permits detection of DNA targets using only two components: a molecular beacon and a site-specific DNA nickase without deoxyribonucleotide triphosphates and primers. The loop sequence of the molecular beacon should contain a DNA nickase recognition site. The nickase–molecular beacon (NMB) combination permits a 100-fold increase in fluorescent signal. The applications of the NMB assay for enhancement of fluorescent signal in some isothermal methods are discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ludmila A. Zheleznaya, Damir S. Kopein, Evgeniy A. Rogulin, Sergey I. Gubanov, Nikolay I. Matvienko,