Spectrophotometric estimation of functional groups on microslides for preparation of biochips

A universal reagent 1-O-(4,4′-dimethoxytrityl)-6-aminohexanol (DTAH) is described for the estimation of surface-bound functionalities (epoxy, aldehyde, and carboxyl) required for preparation of oligonucleotide arrays (biochips). The method involves the reaction of universal reagent DTAH with surface-bound functionality under microwaves for 10 min, followed by washings to remove the excess reagent. In the subsequent step, a weighed amount of DTAH-treated surface is exposed to acid to liberate 4,4′-dimethoxytrityl cation, which is measured at 505 nm to determine the functional group loading on the surface.