| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1176838 | Analytical Biochemistry | 2006 | 7 Pages |
Abstract
Human nonpancreatic secreted phospholipase A2 (hnps PLA2) is considered to be an important drug target for antiinflammation therapy. We have established a new fluorescence assay by using 1-anilinonaphthalene-8-sulfonate (ANS) as an interfacial probe for hydrophobic environment detection. The fitted apparent kcat/Km of hnps PLA2 is 0.0181 ± 0.0005 RFU/μM s. Tests on known synthesized inhibitor gave IC50 values similar to those from isotope-labeled assay. Because ANS is a commonly used probe for hydrophobic environment detection that needs no modification in the current assay, this strategy may be widely applicable for interfacial catalytic reactions.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Changkang Huang, Lu Zhou, Ying Liu, Luhua Lai,