| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1176972 | Analytical Biochemistry | 2010 | 4 Pages |
This work studied the possibilities for quantitative determination of iron mobilization in connection with ferritin reduction by ascorbic acid (vitamin C) and sodium dithionite in vitro. The iron storage protein was incubated with an excess of reductant in aerobic conditions in the absence of complexing agents in the medium. The release of Fe2+ was let to go to completion, and the overall content of Fe2+ in the solution was evaluated with the aid of potentiometric titration using Ce4+ as an oxidizing titrant. Results suggest a moderate iron efflux under the influence of the chosen reducing agents. Although such a reduction of the protein mineral core by dihydroxyfumarate contributes greatly to the iron mobilization, ferritin behavior with vitamin C and dithionite seems to be different. Although redox properties of dihydroxyfumarate are determined by hydroxyl groups similar to those of ascorbic acid, the two compounds differ significantly in structure, and this could be the basis for an explanation of the specificities in their interaction with ferritin. As revealed by the study, potentiometric titration promises to be a reliable tool for evaluation of the amount of Fe2+ present in the solution as a result of the reduction of the ferritin’s mineral core.
