Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1177161 | Analytical Biochemistry | 2008 | 8 Pages |
Abstract
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yi Long, Xiaocui Xing, Ruifang Han, Yang Sun, Ying Wang, Zhuo Zhao, Huaifeng Mi,