Article ID Journal Published Year Pages File Type
1177161 Analytical Biochemistry 2008 8 Pages PDF
Abstract
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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