| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1177313 | Analytical Biochemistry | 2007 | 6 Pages |
Abstract
An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Jeremiah W. Hanes, Clifford E. Kraft, Tadhg P. Begley,