Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1177354 | Analytical Biochemistry | 2007 | 9 Pages |
Abstract
Double-stranded calf thymus (dsCT)-DNA was electrochemically entrapped into polypyrrole-polyvinyl sulfonate (PPy-PVS) films deposited onto indium tin oxide (ITO) coated glass plates. These dsCT-DNA entrapped PPy-PVS/ITO films were characterized using cyclic voltammetry, UV-visible, Fourier transform infrared (FT-IR), scanning tunneling microscopy (STM), and electrochemical impedance measurements. Attempts made to use these dsCT-DNA entrapped PPy-PVS/ITO films for detection of 2-aminoanthracene (0.001-6.0 ppm) and 3-chlorophenol (0.01-55.0 ppm) revealed a response time of 30 s and a shelf life of approximately 25 weeks when stored under desiccated conditions at 25 °C. The addition of salts such as Ca2+ (250 ppm), Mg2+ (200 ppm), Clâ (1560 ppm), and Na+ (150 ppm) ions contained in water does not affect the observed amperometric response of the disposable dsCT-DNA entrapped PPy-PVS film-based electrochemical biosensor.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nirmal Prabhakar, Kavita Arora, Surinder P. Singh, Harpal Singh, Bansi D. Malhotra,