| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1177375 | Analytical Biochemistry | 2007 | 7 Pages |
Abstract
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Evgenia G. Matveeva, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R. Lakowicz, Zygmunt Gryczynski,