Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1177481 | Analytical Biochemistry | 2006 | 7 Pages |
Abstract
The activity of the pyruvate dehydrogenase complex has long been determined in some laboratories by coupling the production of acetyl-coenzyme A (acetyl-CoA) to the acetylation of 4-aminoazobenzene-4′-sulfonic acid by arylamine N-acetyltransferase. The assay has some advantages, but its use has been limited by the need for large amounts of arylamine N-acetyltransferase. Here we report production of recombinant chicken liver arylamine N-acetyltransferase and optimization of its use in miniaturized assays for the pyruvate dehydrogenase complex and its kinase.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nam Ho Jeoung, Paresh C. Sanghani, Lanmin Zhai, Robert A. Harris,