| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1177487 | Analytical Biochemistry | 2006 | 6 Pages |
Abstract
We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mathias Hampf, Manfred Gossen,