Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1177492 | Analytical Biochemistry | 2006 | 10 Pages |
Abstract
A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Andrey Semenyuk, Matilda Ahnfelt, Camilla Estmer Nilsson, Xiao Yong Hao, Andras Földesi, Yu-Shu Kao, Hong-Huei Chen, Wei-Chen Kao, Konan Peck, Marek Kwiatkowski,