Article ID Journal Published Year Pages File Type
1177825 Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2015 14 Pages PDF
Abstract

•The nervous system in crustacean is an excellent model for neuropeptide study.•Detailed workflows of MS imaging and microdialysis in crustacean are discussed.•Advances in neuropeptide analysis at the spatial and temporal domains are noted.•MS-based techniques provide comprehensive information of crustacean neuropeptides.

Neuropeptides represent one of the largest classes of signaling molecules used by nervous systems to regulate a wide range of physiological processes. Over the past several years, mass spectrometry (MS)-based strategies have revolutionized the discovery of neuropeptides in numerous model organisms, especially in decapod crustaceans. Here, we focus our discussion on recent advances in the use of MS-based techniques to map neuropeptides in the spatial domain and monitoring their dynamic changes in the temporal domain. These MS-enabled investigations provide valuable information about the distribution, secretion and potential function of neuropeptides with high molecular specificity and sensitivity. In situ MS imaging and in vivo microdialysis are highlighted as key technologies for probing spatio-temporal dynamics of neuropeptides in the crustacean nervous system. This review summarizes the latest advancement in MS-based methodologies for neuropeptide analysis including typical workflow and sample preparation strategies as well as major neuropeptide families discovered in decapod crustaceans. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.

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Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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