| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1178219 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2016 | 9 Pages |
•ProTSAV delivers an effective combination of modules and outclasses their discrete accuracies.•Analysis from all available resources results in better insights into input protein structure.•Its high sensitivity and specificity for model structures assure reliable and accurate quality assessment.•User is furnished with an easy to interpret result in the form of a graphical representation.
Quality assessment of predicted model structures of proteins is as important as the protein tertiary structure prediction. A highly efficient quality assessment of predicted model structures directs further research on function. Here we present a new server ProTSAV, capable of evaluating predicted model structures based on some popular online servers and standalone tools. ProTSAV furnishes the user with a single quality score in case of individual protein structure along with a graphical representation and ranking in case of multiple protein structure assessment. The server is validated on ~ 64,446 protein structures including experimental structures from RCSB and predicted model structures for CASP targets and from public decoy sets. ProTSAV succeeds in predicting quality of protein structures with a specificity of 100% and a sensitivity of 98% on experimentally solved structures and achieves a specificity of 88%and a sensitivity of 91% on predicted protein structures of CASP11 targets under 2 Å.The server overcomes the limitations of any single server/method and is seen to be robust in helping in quality assessment.ProTSAV is freely available at http://www.scfbio-iitd.res.in/software/proteomics/protsav.jsp
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (416 K)Download as PowerPoint slideProTSAV brings an efficient unification of available resources for quality assessment and outperforms their individual accuracies. By means of ProTSAV, a reliable and accurate quality assessment of model structures for applicability in different fields is facilitated. It furnishes the user with an easy to interpret result in the form of a graphical representation.
