Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1178247 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2008 | 5 Pages |
Abstract
Insulin degrading enzyme (IDE) is known to play a pivotal role on amyloidogenic peptide degradation but little is known about the changes in the proteolytic activity of the enzyme upon modification of external factors. Particularly, although it has been reported that altered ubiquitin concentration and/or hyperinsulinaemia increase the risk of developing Alzheimer's disease (AD), the molecular mechanism involved is unclear. In this work, we study the role that ubiquitin plays on IDE capability of binding and degrading insulin molecules and the obtained results indicate that ubiquitin has an allosteric role for IDE and high ubiquitin levels impair IDE activity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Giuseppe Grasso, Enrico Rizzarelli, Giuseppe Spoto,