| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1178553 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2012 | 8 Pages |
Abstract
⺠We investigated the role of disulfide bonds in stability and folding of SsMTAPII. ⺠We performed the spectroscopic characterization of SsMTAPII and its mutants. ⺠We carried out thermal and GdmCl-induced unfolding/refolding experiments. ⺠Chemical and thermal unfolding are reversible only under reducing conditions. ⺠Unfolding occurs with dissociation of the native hexamer into denatured monomers.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Giovanna Cacciapuoti, Francesca Fuccio, Luigi Petraccone, Pompea Del Vecchio, Marina Porcelli,