Acclimation increases freezing stress response of Arabidopsis thaliana at proteome level

This study used 2DE to investigate how Arabidopsis thaliana modulates protein levels in response to freezing stress after sub-lethal exposure at − 10 °C, both in cold-acclimated and in non-acclimated plants. A map was implemented in which 62 spots, corresponding to 44 proteins, were identified. Twenty-two spots were modulated upon treatments, and the corresponding proteins proved to be related to photosynthesis, energy metabolism, and stress response. Proteins demonstrated differences between control and acclimation conditions. Most of the acclimation-responsive proteins were either not further modulated or they were down-modulated by freezing treatment, indicating that the levels reached during acclimation were sufficient to deal with freezing. Anabolic metabolism appeared to be down-regulated in favor of catabolic metabolism. Acclimated plants and plants submitted to freezing after acclimation showed greater reciprocal similarity in protein profiles than either showed when compared both to control plants and to plants frozen without acclimation. The response of non-acclimated plants was aimed at re-modulating photosynthetic apparatus activity, and at increasing the levels of proteins with antioxidant-, molecular chaperone-, or post-transcriptional regulative functions. These changes, even less effective than the acclimation strategy, might allow the injured plastids to minimize the production of non-useful metabolites and might counteract photosynthetic apparatus injuries.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (228 K)Download as PowerPoint slideHighlights► Freezing tolerance in acclimated and non-acclimated Arabidopsis thaliana was analyzed. ► Previous acclimation gives more resistance to the subsequent freezing stress. ► Functional modulation of photosynthetic apparatus is showed. ► Down-regulation of anabolic proteins is involved in the survival strategy. ► When not acclimated, plants show weaker response.