Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1178925 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2010 | 9 Pages |
Abstract
Peptide XT-7 (GLLGP5LLKIA10AKVGS15NLL.NH2) is a cationic, leucine-rich peptide, first isolated from skin secretions of the frog, Silurana tropicalis (Pipidae). The peptide shows potent, broad-spectrum antimicrobial activity but its therapeutic potential is limited by haemolytic activity (LC50 = 140 µM). The analogue [G4K]XT-7, however, retains potent antimicrobial activity but is non-haemolytic (LC50 > 500 µM). In order to elucidate the molecular basis for this difference in properties, the three dimensional structures of XT-7 and the analogue have been investigated by proton NMR spectroscopy and molecular modelling. In aqueous solution, both peptides lack secondary structure. In a 2,2,2-trifluoroethanol (TFE-d3)-H2O mixed solvent system, XT-7 is characterised by a right handed α-helical conformation between residues Leu3 and Leu17 whereas [G4K]XT-7 adopts a more restricted α-helical conformation between residues Leu6 and Leu17. A similar conformation for XT-7 in 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micellular media was observed with a helical segment between Leu3 and Leu17. However, differences in side chain orientations restricting the hydrophilic residues to a smaller patch resulted in an increased hydrophobic surface relative to the conformation in TFE-H2O. Molecular modelling of the structures obtained in our study demonstrates the amphipathic character of the helical segments. It is proposed that the marked decrease in haemolytic activity produced by the substitution Gly4 â Lys in XT-7 arises from a decrease in both helicity and hydrophobicity. These studies may facilitate the development of potent but non-toxic anti-infective agents based upon the structure of XT-7.
Keywords
Silurana tropicalisRMSDMICNOENOESYTOCSYdHPCtrifluoroethanolTFEnuclear magnetic resonance1,2-dihexanoyl-sn-glycero-3-phosphocholineNuclear Overhauser Effect SpectroscopYAmphipathic α-helixnuclear overhauser effectNMRMinimum inhibitory concentrationcircular dichroismTotal correlation spectroscopyAntimicrobialroot mean square deviation
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Anusha P. Subasinghage, J. Michael Conlon, Chandralal M. Hewage,