Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1179221 | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics | 2007 | 13 Pages |
Abstract
The antimicrobial peptide esculentin 1-21 (Esc 1-21) is a shorter synthetic version of the 46-residue peptide occurring in the Rana esculenta skin secretion. Here we propose an integrated proteomic and transcriptomic approach to interpret the biological effects of this peptide on Saccharomyces cerevisiae. We further investigated the response to this peptide by correlating the results of the transcriptome and proteome analysis with phenotypic effects. The results show that S. cerevisiae adapts to Esc 1-21 using the High Osmolarity Glycerol (HOG) pathway involved in osmotic tolerance and cell wall maintenance. Comparative proteomics reveals that Esc 1-21 causes downregulation of enzymes of the lower glycolytic pathway and in genes involved in spindle body formation and remodelling of cell-wall synthesis. Moreover the peptide induces downexpression of protein actin within 45Â min and cells pre-treated with peptide show less sensitivity to osmotic stress and increased sensitivity to heat shock stress. The results obtained with the two different methodologies are in agreement at the cellular process levels. A combined approach may help elucidate the main aspects related to the effects of this peptide on the eukaryotic cell. The employment of different technologies may reveal the potential and limitations of each adapted approach in a prospective application for drug screening.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tania Gamberi, Duccio Cavalieri, Francesca Magherini, Maria L. Mangoni, Carlotta De Filippo, Marina Borro, Giovanna Gentile, Maurizio Simmaco, Alessandra Modesti,