Article ID Journal Published Year Pages File Type
1179539 Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014 10 Pages PDF
Abstract

•D. vulgaris AhbD performs the last step of the alternative haem b biosynthesis.•AhbD promotes two oxidative decarboxylations of iron-coproporphyrin III.•AhbD cleaves SAM to produce methionine and 5′-deoxyadenosyl-radical.•AhbD is a SAM radical enzyme that contains two [4Fe–4S]2 +/1 + centres.

An alternative route for haem b biosynthesis is operative in sulfate-reducing bacteria of the Desulfovibrio genus and in methanogenic Archaea. This pathway diverges from the canonical one at the level of uroporphyrinogen III and progresses via a distinct branch, where sirohaem acts as an intermediate precursor being converted into haem b by a set of novel enzymes, named the alternative haem biosynthetic proteins (Ahb). In this work, we report the biochemical characterisation of the Desulfovibrio vulgaris AhbD enzyme that catalyses the last step of the pathway. Mass spectrometry analysis showed that AhbD promotes the cleavage of S-adenosylmethionine (SAM) and converts iron-coproporphyrin III via two oxidative decarboxylations to yield haem b, methionine and the 5′-deoxyadenosyl radical. Electron paramagnetic resonance spectroscopy studies demonstrated that AhbD contains two [4Fe–4S]2 +/1 + centres and that binding of the substrates S-adenosylmethionine and iron-coproporphyrin III induces conformational modifications in both centres. Amino acid sequence comparisons indicated that D. vulgaris AhbD belongs to the radical SAM protein superfamily, with a GGE-like motif and two cysteine-rich sequences typical for ligation of SAM molecules and iron-sulfur clusters, respectively. A structural model of D. vulgaris AhbD with putative binding pockets for the iron-sulfur centres and the substrates SAM and iron-coproporphyrin III is discussed.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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