Localised interventions in cellular processes

The once linear view of cell regulatory processes is now changing as we begin to overlay spatial and temporal characteristics onto signalling pathways and dynamic membranous events. To better understand the properties of these spatially restricted processes we must refine our targeting of these events with acute localised manipulations. We review here the diverse application of a dimerisation system, which exploits immunosuppressor/immunophilin biology to provide a route to drug-inducible subdomain interventions. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases (2012).

► Collective evidence for a localised intervention strategy ► Application to spatially organised signalling pathways and processes ► Commentary on the application in various cell biological systems ► Prospects for signalling sufficiency and impact on future pathway inhibition