Article ID Journal Published Year Pages File Type
1181178 Chemometrics and Intelligent Laboratory Systems 2009 8 Pages PDF
Abstract

Analysis of proteomic data requires the alignment of 2D gel electropherograms. The Matlab ‘Image Processing Toolbox’ contains many interesting subroutines to deal with image processing. Among them there is a subroutine for manual markers selection. Attractive in many cases, it is time-consuming and error prone in the case of gel images, which contain thousands of spots with unknown correspondence. Replacing this subroutine with a fuzzy warping algorithm allows efficient automatic identification of corresponding features. In this paper the principles of image alignment are described and demonstrated using real examples.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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