Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1181508 | Chemometrics and Intelligent Laboratory Systems | 2009 | 14 Pages |
Abstract
Based on a recently developed spot segmentation method, we here present a new approach to modelling of individual spots in digital images, e.g. images of DNA microarrays. From the model parameter estimates and residuals we have developed an expedient approach to automatic quality assessment and identification of corrupted spots. The suggested approach to quality control is shown to give a statistically significant decrease of the variance in gene expression log-ratio estimates for three different DNA microarray datasets.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ãystein Gjerstad, Ã
got Aakra, Ulf Indahl,