| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1181846 | Chinese Journal of Analytical Chemistry | 2016 | 8 Pages |
Separation of active components from Traditional Chinese medicine (TCM) is always difficult due to its complex matrix. The prep-HPLC-based multidimensional chromatography, which combined the characteristics of different separation techniques to improve separation capability and efficiency, is more conductive for separating and purifying complex TCMs. In this paper, we reviewed the basic principles, separation mode, key techniques of prep-HPLC-based multidimensional chromatography and its application in TCM research.
Graphical abstractIn a preparative HPLC-based multidimensional chromatography system, crude drug or crude isolates that prepared by column chromatography or other technical is purified on the 1D-LC. Then a further purification is performed on the 2D-LC for those unseparated target component by off-line or on-line mode.Figure optionsDownload full-size imageDownload as PowerPoint slide
