Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone

To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, a Eu3+ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The experimental conditions were optimized as follows: the AMOZA-OVA concentration was 0.25 μg mL−1; the antibody was diluted by 50000 folds, and the competitive reaction time was 50 min. Under the optimal conditions, the method showed a detection limit of 0.01 ng mL−1, an IC50 of 0.26 ng mL−1 and a linear range (IC20−IC80) of 0.025−2.83 ng mL−1. The spiked recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suitable for the determination of AMOZ residue in food samples.

Graphical abstractAn indirect competitive time-resolved fluoroimmunoassay (ic-TRFIA) for the determination of furaltadone metabolite AMOZ was developed with high accuracy and sensitivity by using Eu3+ as tracer. The ic-TRFIA method was successfully applied to the detection of AMOZ residue in fish sample with satisfactory results.Figure optionsDownload full-size imageDownload as PowerPoint slide