| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1181984 | Chinese Journal of Analytical Chemistry | 2010 | 4 Pages |
Abstract
A novel method to prepare Fe3+ immobilized metal affinity chromatography monolithic column was developed, which included in situ polymerization of N-acryloxysuccinimide and ethylene dimethacrylate, nitrilotriacetic acid activation, and Fe3+ immobilization. The monolithic column showed high selectivity, high binding capacity, good recovery, and good reproducibility for the enrichment of phosphopeptides. Such a column was successfully applied for the enrichment of phosphopeptides from the nonfat milk protein digests. All these results show the promising potential of monolithic column for phosphoproteome analysis.
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