Determination of Nɛ-Carboxymethyl-lysine Content in Muscle Tissues of Turbot by Gas Chromatography-Mass Spectrometry

A method was developed to determine the Nɛ-carboxymethyl-lysine content in the muscle tissues of turbot using gas chromatography-mass spectrometric method. Turbot was selected as the research object, and homogenized muscle tissue samples of turbot defatted by chloroform/acetone were pretreated followed by reduction, acid hydrolysis, purifying and derivatization (esterification and acylation). The ion of m/z 392 was selected as a quantitative ion, and the ions of m/z 392, 365, 333, and 305 were selected as qualitative ions in the selected ion monitoring mode. The range of the linear calibration curve of the Nɛ-carboxymethyl-lysine derivative peak area and concentration extended from 1 to 200 μg L−1. The limit of detection was 1 mg kg−1, and the limit of quantification was 5 mg kg−1. The average recoveries ranged from 100% to 104% at 10, 150 and 350 mg kg−1 spiked levels, and the relative standard deviation was within 5% (n = 5).

Graphical abstractA GC-MS method was developed to determine CML content in turbot muscle tissue. The detection limit was 1 mg kg−1, with the concentration ranged from 1 to 200 μg L−1. As compared with ELISA and other methods reported previouly, GC-MS avoided false positives and could be used to analyze the CML content more accurately.Figure optionsDownload full-size imageDownload as PowerPoint slide