Two-dimensional Nuclear Magnetic Resonance Spectroscopy with Parallel Acquisition of 1H-1H and 19F-19F Correlations

A pulse sequence was established to obtain the two-dimensional nuclear magnetic resonance (NMR) spectroscopy with parallel acquisition of 1H-1H and 19F-19F correlations (1H/19F PANSY-COSY) using a 600 MHz Agilent Direct Drive II NMR instrument equipped with a triple resonance HFX probe feeding 1H and 19F receiver channels independently. The results of homo-nuclear COSY experiments on 4,4,5,5,6,6,7,7,7-nonafluoro-1-heptanol provided simultaneously 1H-1H and 19F-19F correlation spectra, which were compared with the regular COSY spectra acquired under the same conditions. The 1H/19F PANSY-COSY data showed the same resolution and signal sensitivity as the regular 1H-1H COSY and 19F-19F COSY while saving 40% of the experiment time. To improve the resolution for 1H/19F PANSY-COSY signals along the indirectly detection dimension, a narrow spectral window was set up in favor of the 1H chemical shifts range. The resulted 19F-19F COSY signals were folded along the indirectly detection dimension, and the spectra quality was improved.

Graphical abstractA novel pulse sequence was established to obtain 2D-NMR spectroscopy with parallel acquisition of 1H-1H and 19F-19F correlations (1H/19F PANSY-COSY). The 1H/19F PANSY-COSY result of 4,4,5,5,6,6,7,7,7-nonafluoro-1-heptanol showed the same resolution and signal sensitivity as the regular 1H-1H COSY and 19F-19F COSY but saving 40% of the experiment time.Figure optionsDownload full-size imageDownload as PowerPoint slide