| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1182027 | Chinese Journal of Analytical Chemistry | 2010 | 4 Pages |
Abstract
To optimize the 18O-labeling method, two key aspects, peptide dispersion and trypsin deactivation, were discussed. The addition of RapiGest™ SF in H218O and microwave heating enhanced labeling efficiency of α-casein digested peptides (18O/16O ratio > 99%). Chemical modification with tris (2-carboxyethyl) phosphine and iodoacetamide resulted in trypsin deactivation completely. No significant back-exchange from 18O to 16O was observed after labeled in 6 days. The experiment result with peptide mixture from thyroglobulin showed that the improved method could be effectively used to label protein and peptide.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
