Optimal Control of Voltages on Two-dimensional Electrophoresis Microchips

Based on the construction characteristics of two-dimensional microfluidic electrophoretic chips (2D-EMC), an equivalent resistance model of the microchips was established to calculate the rational ranges of voltages applied in the microchip reservoirs. Through the adjustment or optimization of electrophoretic voltages according to the measurements of electroosmotic flows, a group of optimal control voltages was obtained from the reasonable value scope of the reservoir voltages with the equivalent resistance model. The optimized control voltages were successfully applied to the two-dimensional separations of Arg and Gly derivatives on 2D-EMC with chemiluminescence detection. The experiment results indicate that this method significantly reduced the trial times needed for optimizing electrophoretic voltages of 2D-EMC separation.