Study of Liposome Electrofusion on Microelectrode Array Chip

Large liposomes were prepared by using soybean lecithin and the co-evaporation method, and they were electrofused on a microelctrode-array based microchip. In the electrofusion process, the liposomes were aligned by the dielectrophoretic force, electroporated by using high-strength electric pulses, and subsequently fused by using continuous dielectrophoretic force. The experimental results showed that 20% liposomes could be fused on the chip, and the glass substrate and low depth-width ratio channel were helpful for the observation and control of the liposome fusion.